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Solid-state 13C NMR investigations of the glycosidic linkage in α-α′ trehalose

✍ Scribed by P Zhang; A.N Klymachyov; S Brown; J.G Ellington; P.J Grandinetti

Book ID
104357982
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
220 KB
Volume
12
Category
Article
ISSN
0926-2040

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✦ Synopsis

The sugar trehalose is known to play a central role in the desiccation tolerance of many organisms. Essential to trehalose's role are its glass forming abilities and ability to directly interact with lipid molecules. Detailed information on the structure and dynamics of glassy trehalose and its interactions with lipids, however, have been elusive. We have used Ž . solid-state NMR and ab initio quantum mechanical methods Gaussian 94 in order to characterize the possible molecular Ž Ž . . conformations of trehalose. Using a simplified structure 2-tetrahydropyran-2-yloxy tetrahydropyran as a model we have calculated the energy and 13 C magnetic shielding parameters as a function of the two glycosidic torsion angles. Combining ab initio derived maps and using the 13 C lineshape as constraints we were able to construct the torsion angle distribution map for a-a X trehalose. We believe measurements of 13 C isotropic chemical shift and other solid-state NMR tensor parameter distributions in combination with ab initio methods can prove useful in identifying sources of structural disorder in glassy trehalose. By monitoring these structural distributions new information about the membrane surface associative properties of trehalose and other sugars should be accessible.

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