Conformation of the Glycosidic Linkage in a Disaccharide Investigated by Double-Quantum Solid-State NMR

✍ Scribed by S. Ravindranathan; T. Karlsson; K. Lycknert; G. Widmalm; M.H. Levitt

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 116 KB
• Volume: 151
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.2001.2357

✦ Synopsis

Double-quantum heteronuclear local field NMR is performed on a sample of a 13C2-labeled disaccharide, in which the two 13C spins are located on opposite sides of the glycosidic linkage. The evolution of the double-quantum coherences is found to be consistent with the solid-state conformation of the molecule, as previously determined by X-ray diffraction. The dependence of the double-quantum evolution on the glycosidic torsional angles is examined by using a graphical molecular manipulation program interfaced to a numerical spin simulation module.