Solid-phase ‘phosphite’ synthesis of oligonucleotides
✍ Scribed by Krishna Jayaraman; Hoge McClaugherty
- Book ID
- 107858341
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- French
- Weight
- 165 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
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A general procedure for the synthesis of branched RNA and DNA oligonucleotides has been developed. The synthetic strategy involves the 3'->5' synthesis of a DNA or RNA oligomer on a solid-phase, controlled-pore glass support with an automated DNA synthesizer. The branch point nucleoside is introduce
Summarv-A 5'-O-monomethoxytritylthymidine-3'-S-thiophosphoramidite (3) has been used to prepare oligodeoxynucleotides containing 3'-thiothymidine on a solid phase support. The intermediate thiophosphites are most efficiently oxidised using tetrabutylammonium periodate. Polynucleotide analogues conta