Automated solid-phase synthesis of branched oligonucleotides

✍ Scribed by Masad J. Damha; Steve Zabarylo

• Publisher: Elsevier Science
• Year: 1989
• Tongue: French
• Weight: 205 KB
• Volume: 30
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(01)93876-6

✦ Synopsis

A general procedure for the synthesis of branched RNA and DNA oligonucleotides has been developed. The synthetic strategy involves the 3'->5' synthesis of a DNA or RNA oligomer on a solid-phase, controlled-pore glass support with an automated DNA synthesizer. The branch point nucleoside is introduced by coupling of two adjacent polymer bound nucleotide chains with a tetrazole-activated adenosine 2',3'-bisphosphoramidite derivative.

Branched ribonucleic acids containing vicinal 2'-5' and 3'-5' phosphodiester linkages were first detected in nuclear polyadenylated RNA from HeLa cells by Wallace and Edmonsl and were subsequently shown by others to form an integral part of messenger RNA splicing2. Two unique branched RNA