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Solid phase glycosidation of oligonucleotides

✍ Scribed by Matteo Adinolfi; Gaspare Barone; Lorenzo De Napoli; Luigi Guariniello; Alfonso Iadonisi; Gennaro Piccialli

Book ID
104261023
Publisher
Elsevier Science
Year
1999
Tongue
French
Weight
216 KB
Volume
40
Category
Article
ISSN
0040-4039

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✦ Synopsis

CPG-bound nucleosides (deoxyguanosine excepted) can be stereoselectively glycosylated in high yield at their 5'-hydroxyl by a "disarmed" trichioroacetimidate donor in the presence of stoichiometric amounts of TMSOTf (5 eq.) and in short reaction times. These results allowed the solid-phase synthesis of an oligonucleotide functionalized at both ends with sugar residues.

πŸ“œ SIMILAR VOLUMES

Automated solid-phase synthesis of branc
Automated solid-phase synthesis of branched oligonucleotides
✍ Masad J. Damha; Steve Zabarylo πŸ“‚ Article πŸ“… 1989 πŸ› Elsevier Science 🌐 French βš– 205 KB

A general procedure for the synthesis of branched RNA and DNA oligonucleotides has been developed. The synthetic strategy involves the 3'->5' synthesis of a DNA or RNA oligomer on a solid-phase, controlled-pore glass support with an automated DNA synthesizer. The branch point nucleoside is introduce