𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation of digitalis cardenolides by HPLC

✍ Scribed by Linley, P. A. ;Mohamed, A. G. M.

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
243 KB
Volume
4
Category
Article
ISSN
0935-6304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The separation of Digitalis cardenolides has been carried out by HPLC on an adsorbent column. By choice of suitable mobile phase, isocratic elution permitted resolution of mixtures of (a) aglycones, (b) secondary glycosides, and (c) primary glycosides, while gradient elution provided a means of resolving more complex mixtures of these cardiac steroids. HPLC could therefore be used in the quality control of cardiotonic drugs replacing the TLC tests for related compounds currently used, and by suitable calibration could replace the colorimetric assay procedure normally used for such drugs.

πŸ“œ SIMILAR VOLUMES

Studies on growth and cardenolide produc
Studies on growth and cardenolide production of Digitalis lanata tissue cultures
✍ U. Tuominen; L. Toivonen; V. Kauppinen; P. Markkanen; L. BjΓΆrk πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 353 KB

Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides. The possibility of using a statistical (Box-Wilson) experimental design to study the effects of four different variables on growth, di

Isomers of uroporphyrin free acids separ
Isomers of uroporphyrin free acids separated by HPLC
✍ Wayne, A. W. ;Straight, R. C. ;Wales, E. E. ;Englert, E. πŸ“‚ Article πŸ“… 1979 πŸ› John Wiley and Sons 🌐 English βš– 183 KB

## Abstract A synthetic mixture of uroporphyrin isomers I, II, III and IV as free acids in the synthetic ratio of 1:1:4:2 was resolved by reverse‐phase HPLC using a C0:PEL (ODS) 37‐50 micron precolumn and a Micro Bondapak C18 analytical column eluted with acetonitrile (4%) in phosphate buffer (pH 6

Improved separation of glycosylated tryp
Improved separation of glycosylated tryptic peptides by RP-HPLC
✍ Basak, S. ;Compans, R. W. πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 254 KB

## Abstract The potential of reverse‐phase high‐performance liquid chromatography (RP‐HPLC) for the separation of the tryptic glycopeptides of the hemagglutinin (HA) glycoprotein of influenza virus has been investigated. Excellent separation was accomplished with sample recovery of at least 90‐95%

The optimized separation of aromatic car
The optimized separation of aromatic carboxylic acids by HPLC
✍ Patel, B. ;Purnell, J. H. ;Wellington, C. A. πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 422 KB

The optimization of the separation of eight aromatic carboxylic acids, using Hypersil SAS and MOS reverse phase columns, with respect to eluent (methanol :water) composition, pH, and temperature, is described. Window diagram techniquesareused and the pH of the aqueous buffer and elution temperature