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Isomers of uroporphyrin free acids separated by HPLC

✍ Scribed by Wayne, A. W. ;Straight, R. C. ;Wales, E. E. ;Englert, E.

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
183 KB
Volume
2
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

A synthetic mixture of uroporphyrin isomers I, II, III and IV as free acids in the synthetic ratio of 1:1:4:2 was resolved by reverse‐phase HPLC using a C0:PEL (ODS) 37‐50 micron precolumn and a Micro Bondapak C18 analytical column eluted with acetonitrile (4%) in phosphate buffer (pH 6.95). Clinically important I and III isomers of uroporphyrin were readily resolved directly from acidified urine as porphyrin free acids.

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