The optimized separation of aromatic carboxylic acids by HPLC
β Scribed by Patel, B. ;Purnell, J. H. ;Wellington, C. A.
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 422 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
The optimization of the separation of eight aromatic carboxylic acids, using Hypersil SAS and MOS reverse phase columns, with respect to eluent (methanol :water) composition, pH, and temperature, is described. Window diagram techniquesareused and the pH of the aqueous buffer and elution temperature are shown to be more critical than eluent composition.
π SIMILAR VOLUMES
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The separation and deterrmnatlon of the major carboxyhc acids m wme by hqmd chromatography were optlmlzed by usmg the modelhng surface response method The chromatographlc response functton was used as a crttenon to obtam an optimum peak separation with the munmum analysis time accepted The startmg m