𝔖 Bobbio Scriptorium
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Studies on growth and cardenolide production of Digitalis lanata tissue cultures

✍ Scribed by U. Tuominen; L. Toivonen; V. Kauppinen; P. Markkanen; L. Björk

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
353 KB
Volume
33
Category
Article
ISSN
0006-3592

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✦ Synopsis

Digitalis lanata cell cultures grown as small undifferentiated aggregates in suspension culture can be redifferentiated into green embryos that produce cardenolides. The possibility of using a statistical (Box-Wilson) experimental design to study the effects of four different variables on growth, differentiation, and cardenolide production of D. lanata tissue cultures are investigated. The results of the analyses were processed by linear regression analysis. Mathematical models explaining the effects of the variables were developed. The concentration of maltose and the NO3--NH4' ratio were found to be significant variables for both growth and cardenolide production. The size of the inoculum was also important.

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