𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Improved separation of glycosylated tryptic peptides by RP-HPLC

✍ Scribed by Basak, S. ;Compans, R. W.

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
254 KB
Volume
4
Category
Article
ISSN
0935-6304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The potential of reverse‐phase high‐performance liquid chromatography (RP‐HPLC) for the separation of the tryptic glycopeptides of the hemagglutinin (HA) glycoprotein of influenza virus has been investigated. Excellent separation was accomplished with sample recovery of at least 90‐95% by the utilization of an octadecasilyl silica stationary phase and a phosphoric acid/n‐propanol solvent system. Eight glycosylated tryptic peptide classes were resolved from the HA glycoprotein of the A/USSR/90/77 virus strain.

📜 SIMILAR VOLUMES

Two-dimensional separation of peptides u
Two-dimensional separation of peptides using RP-RP-HPLC system with different pH in first and second separation dimensions
✍ Martin Gilar; Petra Olivova; Amy E. Daly; John C. Gebler 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 442 KB

## Abstract Two‐dimensional high performance liquid chromatography is a useful tool for proteome analysis, providing a greater peak capacity than single‐dimensional LC. The most popular 2D‐HPLC approach used today for proteomic research combines strong cation exchange and reversed‐phase HPLC. We ha

Selective separation of cationic peptide
Selective separation of cationic peptides from a tryptic hydrolysate of β-lactoglobulin by electrofiltration
✍ Jean-François Lapointe; Sylvie F. Gauthier; Yves Pouliot; Christian Bouchard 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 309 KB

## Abstract Electrofiltration (EF) was used to selectively separate cationic (basic) peptides contained in a tryptic β‐lactoglobulin (β‐LG) hydrolysate, with particular emphasis on the isolation of basic sequence β‐LG 142–148, which is a potential antihypertensive peptide. Both the influence of fee

Separation of N-0437 enantiomers by RP-H
Separation of N-0437 enantiomers by RP-HPLC after pre-column derivatization with D(+)-glucuronic acid
✍ Gerding, T. K. ;Drenth, B. F. H. ;de Zeeuw, R. A. 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 238 KB

adducts are destroyed coelutes with the isooctane. Peaks of ## Short Communications Table 1 retention time 2.63,5.44, and 7.65 minutes are from the reagent. Even though these peaksarequitelarge,theydo not interferewith the analysis of the esters. ## Retention data for tert-butyldimethylsilyl est