Separation of bile acids as their phenacyl esters by high-pressure liquid chromatography

✍ Scribed by Frans Stellaard; David L. Hachey; Peter D. Klein

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 391 KB
• Volume: 87
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90685-1

✦ Synopsis

Bile acids have been separated by high-pressure liquid chromatography. The free acids were derivatized to their phenacyl esters by treatment with triethylamine and a-bromoacetophenone. The stationary phase was a C,,, Partisil ODS column. A dual-solvent, stepwise gradient system was used for the mobile phase. The method is applied to a human bile sample and shows excellent resolution of the dihydroxy bile acid phenacyl esters. Detection limits for pure derivatized bile acids are lo-20 pmol (5-10 ng), except for the cholic acid derivative, which has a detection limit of 265 pmol.