Bile acids LIII: Application of reverse-phase high-pressure liquid chromatography to the analysis of conjugated bile acids in bile samples

✍ Scribed by Roger Shaw; J.Alexander Smith; William H. Elliott

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 382 KB
• Volume: 86
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90768-6

✦ Synopsis

The common conjugated bile acids of deproteinated bile from the human or the rat can be separated by high-pressure liquid chromatography and quantitated within 30 min with a 4-mm x 30-cm "fatty-acid analysis" column (Waters Associates) in 2-propanoV8.8 mM potassium phosphate buffer (pH 2.5) 160:340, coupled to a uv flow detector set at 193-nm wavelength. Detection limits were at least 0.1 nmol for the tauro-conjugates and 0.2 nmol for the glyco-derivatives.