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Bile acids: XLVIII. Separation of conjugated bile acids by high-pressure liquid chromatography

โœ Scribed by Roger Shaw; William H. Elliott

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
496 KB
Volume
74
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Major conjugated bile acids of human bile have been resolved by high-pressure liquid chromatography. The elutions are carried out in two stages on Corasil II or PPorasil columns; first, an alkaline solvent system (Zpropanokethyl acetate/ water/7 N ammonium hydroxide, 260:600:50:3) was used for separation into groups: tauro-dihydroxy derivatives, taurocholate, glyco-dihydroxy derivatives, and glycocholate. The fraction containing glyco-dihydroxy conjugates was separated by rechromatography in acetonitrile/acetic acid, 400:10, and the fraction containing tauro-dihydroxy conjugates could be partially resolved by rechromatography in acetonitrilelacetic acid/formic acid (97%)/water, 500: 105: 10. Three samples of prepared human bile have been similarly treated.

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