Separation of conjugated bile acids by two-dimensional thin-layer chromatography
β Scribed by Levitt, R. E. ;Touchstone, J. C.
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 130 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
Thin-layer chromatography, two dimensional combination of non polar chemical bonded phase / silica gel separation of all bile acids on one chromatogram sensitivity of detection 25 ng for TCDC, GCDC, TDC, GDC, TC, phase GC
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Several methods for evaluating separation quality in two-dimensional thin layer chromatography are discussed. These include using either the distance between spot centers for the pair of solutes that is the most difficult to separate in a mixture, or the value of a suitable separation function, as c
Thin-layer chromatographic systems and spray reagents are described for the rapid differentiation of 10 penicillins. Comparative behavior of 18 other antibiotics under these conditions is presented together with other supporting data.