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Determination of dipicolinic acid by high-pressure liquid chromatography

โœ Scribed by Henry Paulus


Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
296 KB
Volume
114
Category
Article
ISSN
0003-2697

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A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated fr