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Determination of tissue UDP-glucuronic acid levels by high-pressure liquid chromatography

โœ Scribed by Carol M. Schiller; Cindy S. Dieringer; Margaret E. Twine; A.Robert Jeffcoat


Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
381 KB
Volume
127
Category
Article
ISSN
0003-2697

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A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated fr