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Separation and formation of ryanodine from dehydroryanodine. Preparation of tritium-labeled ryanodine

✍ Scribed by John L. Sutko; Lorri J. Thompson; Robert G. Schlatterer; Frank A. Lattanzio; Allan S. Fairhurst; Carlton Campbell; Stephen F. Martin; Pierre Deslongehamps; Luc Ruest; David R. Taylor

Publisher
John Wiley and Sons
Year
1986
Tongue
French
Weight
321 KB
Volume
23
Category
Article
ISSN
0022-2135

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✦ Synopsis

The commercially available preparation of the naturally occurring diterpene ester ryanodine contains several compounds in addition to ryanodine. In the present study these compounds were separated and purified using high performance liquid chromatography. The two major components, ryanodine and dehydroryanodine represented 90% of the material present. A method for the efficient reduction of dehydroryanodine to ryanodine was developed and used to produce ryanodine having tritium atoms at positions 19 and 20 and a specific activity of 60.8 Cifmmole.

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