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The preparation of tritium-labelled ryanodine

✍ Scribed by Alan S. Fairhurst

Publisher: John Wiley and Sons
Year: 1971
Tongue: French
Weight: 203 KB
Volume: 7
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2590070205

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✦ Synopsis

Abstract

A method for preparing tritium‐labelled ryanodine is described. The alkaloid was first brominated, and the bromoryanodines were separated from the reaction mixture by thin layer chromatography; NMR analysis at this stage showed substitution of pyrrole protons by bromine. Bromoryanodine was first hydrogenated, and the product was purified and characterized as ryanodine, to establish the feasibility of the method. Tritium was then reacted with bromoryanodine to yield labelled ryanodine with a specific activity of 2.85 Ci/mmole. No detectable isotope exchange occurred when the labelled alkaloid was incubated in aqueous media at pH 7.0 and 30° C.

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