## Abstract A method for preparing tritium‐labelled ryanodine is described. The alkaloid was first brominated, and the bromoryanodines were separated from the reaction mixture by thin layer chromatography; NMR analysis at this stage showed substitution of pyrrole protons by bromine. Bromoryanodine
The preparation of tritium labelled biocompatible polymers
✍ Scribed by Jeremy C. Russell; John R. Jones; Terrence A. Vick; Peter W. Stratford
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- French
- Weight
- 245 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
The preparation of tritiated phosphoryl choline containing linear polymers by the reduction of an acetylenically unsaturated polymer with tritium gas is described. Homogeneous catalysis was used and the product was analysed by ^1^H and ^3^H‐nmr spectroscopy. The use of lanthanide shift reagents showed that 98% of the isotope was located in the 7 and 8 positions of the hydrophobic chains.
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Tritiated N-chloracetyl-DL-phenylalanine, obtained by reduction of the N-chloracetyl-dehydrophenylalanine with tritiated hydrogen, is hydrolysed by carboxypeptidase A . The method gives L-phenyl-al~nine-2,3-~H of high optical purity and with any specific activity up to 60 curies per millimole.
## Abstract The synthesis of d, l, ‐12‐bromocamptothecin (**2d**) from camptothecin (**1**) is described. Reduction of the bromo derivative **2d** with tritium gas in the presence of palladium on carbon afforded d, l‐camptothecin 12‐^3^H having a specific activity of 29 Ci/mmol. A simpler labelling
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