Selective transfer of IgM from agarose gels to nitrocellulose membranes
β Scribed by Wayne L. Hoffman; Adrien A. Jump
- Book ID
- 118900757
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 103 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0022-1759
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π SIMILAR VOLUMES
The conditions which atfect the elution of polypeptides from polyacrylamide gels by electrophoresis and polypeptide-nitrocellulose interactions have been studied. The rate of elution of polypeptides from a 15% sodium dodecyl sulfate-polyacrylamide gel is dependent on the molecular weight of the indi
A method describing the rapid and effective transfer of integral membrane protein from isoelectric focusing gels to nitrocellulose is described. Initial experiments were carried out with detergentsolubilized extracts of human erythrocyte membrane proteins. The effectiveness of the transfer was demon
A rapid picture of the extent of protein transfer from one-or two-dimensional polyacrylamide gels onto nitrocellulose membranes prior to immunodetection can be obtained by a rapid and reversible Amido Black staining procedure.