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Simple reversible staining of proteins transferred from polyacrylamide gels onto nitrocellulose membranes

โœ Scribed by Hans Bloemendal; Monique Satijn


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
181 KB
Volume
300
Category
Article
ISSN
0003-2670

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โœฆ Synopsis


A rapid picture of the extent of protein transfer from one-or two-dimensional polyacrylamide gels onto nitrocellulose membranes prior to immunodetection can be obtained by a rapid and reversible Amido Black staining procedure.


๐Ÿ“œ SIMILAR VOLUMES


Rapid and effective transfer of integral
โœ Stephan Matthaei; Deborah L. Baly; Richard Horuk ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 493 KB

A method describing the rapid and effective transfer of integral membrane protein from isoelectric focusing gels to nitrocellulose is described. Initial experiments were carried out with detergentsolubilized extracts of human erythrocyte membrane proteins. The effectiveness of the transfer was demon