A method is described for analyzing calcium/calmodulin-dependent protein kinase activity in crude or purified samples separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to PVDF membrane. The blotted protein is denatured in situ with guanidine \
✦ LIBER ✦
Calmodulin binding to nitrocellulose and Zetapore membranes during electrophoretic transfer from polyacrylamide gels
✍ Scribed by Dr. Cécile Rochette-Egly; Danièle Daviaud
- Book ID
- 102833974
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 562 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0173-0835
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A method describing the rapid and effective transfer of integral membrane protein from isoelectric focusing gels to nitrocellulose is described. Initial experiments were carried out with detergentsolubilized extracts of human erythrocyte membrane proteins. The effectiveness of the transfer was demon