𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Calmodulin binding to nitrocellulose and Zetapore membranes during electrophoretic transfer from polyacrylamide gels

✍ Scribed by Dr. Cécile Rochette-Egly; Danièle Daviaud


Book ID
102833974
Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
562 KB
Volume
6
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Renaturation of Calcium/Calmodulin-Depen
✍ D.A. Shackelford; J.A. Zivin 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 824 KB

A method is described for analyzing calcium/calmodulin-dependent protein kinase activity in crude or purified samples separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to PVDF membrane. The blotted protein is denatured in situ with guanidine \

Rapid and effective transfer of integral
✍ Stephan Matthaei; Deborah L. Baly; Richard Horuk 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 493 KB

A method describing the rapid and effective transfer of integral membrane protein from isoelectric focusing gels to nitrocellulose is described. Initial experiments were carried out with detergentsolubilized extracts of human erythrocyte membrane proteins. The effectiveness of the transfer was demon