Transfer of proteins from plastic-backed isoelectric focusing gels to nitrocellulose paper

Rapid and effective transfer of integral

Rapid and effective transfer of integral membrane proteins from isoelectric focusing gels to nitrocellulose membranes

✍ Stephan Matthaei; Deborah L. Baly; Richard Horuk 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 493 KB

A method describing the rapid and effective transfer of integral membrane protein from isoelectric focusing gels to nitrocellulose is described. Initial experiments were carried out with detergentsolubilized extracts of human erythrocyte membrane proteins. The effectiveness of the transfer was demon