Secondary Structure of the lac Repressor Headpiece : Possibilities and Limitations of a Joint Infrared and Circular Dichroism Study
β Scribed by Manfred SCHNARR; Jean-Claude MAURIZOT
- Book ID
- 115121446
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 633 KB
- Volume
- 128
- Category
- Article
- ISSN
- 1432-1327
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## Abstract The __lac__ repressor and its tryptic core were studied by ir spectroscopy, and their Ξ²βstructure content was determined by analysis of the spectra. Using proteinβderived reference spectra, we find a Ξ²βcontent for __lac__ repressor of 18% and of 23% for its tryptic core. The higher amou
Selected regions of infarred (ir) and circular dichroism (CD) spectral data from 10 proteins were combined and analyzed by a factor analysis method. The regions consisted of the area normalized amide I region from 1700 to 1600 cm-1 for the ir spectra and from 178 to 240 nm for the CD spectra. Each C