The optimization of protein secondary structure determination with infrared and circular dichroism spectra
β Scribed by Keith A. Oberg; Jean-Marie Ruysschaert; Erik Goormaghtigh
- Book ID
- 110915141
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 811 KB
- Volume
- 271
- Category
- Article
- ISSN
- 1432-1327
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Vibrational circular dichroism (VCD) provides alternative views of protein and peptide conformation with advantages over electronic (UV) CD (ECD) or IR spectroscopy. VCD is sensitive to short-range order, allowing it to discriminate beta-sheet and various helices as well as disordered structure. Qua
## Abstract New βreferenceβ circular dichroism spectra of Ξ± helix, Ξ²βstructure (both parallel and antiparallel), Ξ²βbends, and the unordered form are obtained from circular dichroism spectra and xβray data for six proteins (myoglobin, lysozyme, lactate dehydrogenase, papain, ribonuclease, and subtil
The estimation of protein secondary structure from circular dichroism spectra is described by a multivariate linear model with noise (Gauss-Markoff model). With this formalism the adequacy of the linear model is investigated, paying special attention to the estimation of the error in the secondary s