Infrared study of the secondary structure of lac repressor and its tryptic core

✍ Scribed by Manfred Schnarr; Jean-Claude Maurizot

• Book ID: 101718092
• Publisher: Wiley (John Wiley & Sons)
• Year: 1980
• Tongue: English
• Weight: 417 KB
• Volume: 19
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1980.360191104

✦ Synopsis

Abstract

The lac repressor and its tryptic core were studied by ir spectroscopy, and their β‐structure content was determined by analysis of the spectra. Using protein‐derived reference spectra, we find a β‐content for lac repressor of 18% and of 23% for its tryptic core. The higher amount of β‐conformation in the tryptic core is confirmed by another type of analysis (decomposition of the spectra in Gaussian curves). These results are discussed with respect to their implications for the structure of the N‐terminal “headpiece” of lac repressor and for the mode of interaction of lac repressor with lac operator.