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Role of the RecF gene product in UV mutagenesis of lambda phage

✍ Scribed by Wood, Richard Dean ;Stein, Judith

Publisher
Springer
Year
1986
Tongue
English
Weight
341 KB
Volume
204
Category
Article
ISSN
0026-8925

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✦ Synopsis

E. coli recF mutants have a greatly reduced capacity for Weigle mutagenesis of ultraviolet light-irradiated lambda phage. A recF 332::Tn3 mutation was introduced into an E. coli recA441 lexA51 strain which constitutively expresses SOS functions. Weigle mutagenesis of phage lambda could occur in the resulting strain in the absence of host cell irradiation, and was increased when the recA441 (tif) allele was activated by increased temperature and excess adenine. The inability of recF strains to support Weigle mutagenesis can therefore be ascribed to a defect in expression of SOS functions after irradiation.

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