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Untargeted mutagenesis induced by UV in the lacI gene of Escherichia coli

✍ Scribed by Christensen, Roshan B. ;Christensen, J. R. ;Koenig, Ian ;Lawrence, Christopher W.

Publisher: Springer
Year: 1985
Tongue: English
Weight: 577 KB
Volume: 201
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00397982

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✦ Synopsis

Using a nonselective method, we have estimated the proportion of untargeted mutations in the lad gene of E. coli by transferring either irradiated or unirradiated F' pro lac plasmids from an excision deficient donor to an excision deficient pro lac deleted recipient that had been irradiated and allowed to induce recA dependent functions for 30 min. We find that about 10 percent of the mutations induced by either 3.5 Jm -2 or 7 Jm -2 UV are untargeted.

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