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Conjugation-dependent enhancement of induced and spontaneous mutation in the lacI gene of E. coli

✍ Scribed by Christensen, Roshan B. ;Christensen, J. R. ;Lawrence, Christopher W.

Publisher: Springer
Year: 1985
Tongue: English
Weight: 339 KB
Volume: 201
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00397983

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✦ Synopsis

The frequency of lac mutations induced in an F'lacI s plasmid, transferred by conjugation from UV-irradiated, excision-deficient donors to excision-deficient, A pro lac recipients, is 2-3 fold higher than that typical of nonmating cells which contain the plasmid. These additional induced mutations can probably be ascribed to errors made during the first, or repliconation, synthesis that takes place in the recipient during the course of plasmid transfer. We also find that spontaneous mutation rates are enhanced in conjugating cells, indicating that fewer errors are corrected, or more made, during transfer replication.

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