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Plasmidic recombination in Escherichia coliK-12: The role of recF gene function

✍ Scribed by Cohen, Amikam ;Laban, Avraham

Publisher: Springer
Year: 1983
Tongue: English
Weight: 412 KB
Volume: 189
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00325911

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✦ Synopsis

Interplasmidic and intraplasmidic recombination proficiencies were determined in E. coli bacterial strains carrying rec mutations. Our results defined the role of recF gene function, recB, recC, and sbcB gene products (exonuclease V and exonuclease I) in plasmidic recombination in wild-type E. coli cells and in cells in which the recE recombination pathway is activated. RecF gene function is required for interplasmidic recombination regardless of the recB recC genotype. Intraplasmidic recombination is recF dependent in cells having a functional exonuclease V, but not in recB recC mutants. Exonuclease V activity inhibits both interplasmidic and intraplasmidic recombination via the recE pathway.

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