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Respiration and glycolysis in the human diploid cell strain wi-38

✍ Scribed by V. J. Cristofalo; David Kritchevsky

Publisher: John Wiley and Sons
Year: 1966
Tongue: English
Weight: 610 KB
Volume: 67
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040670114

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✦ Synopsis

Assessment of the respiratory and glycolytic capacity of non-growing WI-38 cells shows that, in the absence and presence of added glucose, the mean rates of oxygen consumption were 247 (QOz = 5.61) and 208 (QOz = 4.73) mpmoles/mg dry wt/hr., respectively. Mean glucose consumption was 225 mpmoles/mg dry wt/ hr. With uniformly labeled I4C glucose as substrate, 36 mpg atoms of carbon dioxide were produced, corresponding to 15-20% of the total cellular respiration. Mean values for lactate production in the presence and absence of glucose were 345 ( Q 2 = 7.85)

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