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Clone size variation in the human diploid cell strain, wi-38

✍ Scribed by George S. Merz Jr.; John D. Ross

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
377 KB
Volume
82
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

By mapping the location of isolated single cells; and then counting the number of cells at each location as a function of time. it was possible to accumulate data on the growth history for each of a large group of clones. The clone size distribution, its mean and standard deviation were computed for each day in culture. Variations in schedule of medium change and time of exposure to trypsin, did not measurably affect variation in clone size. Neither could clone size variation be accounted for on the basis of (1) occurrence of nondividing cells nor (2) presence of heritable growth rate variants in the population. It is probable that clone size variation under our conditions is primarily a consequence of a highly variable interdivision time among the constituent cells.

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