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The effect of pH on the propagation of the diploid cell WI-38 in galactose medium

✍ Scribed by Clarence L. Baugh; Robert W. Lecher; Alfred A. Tytell

Publisher: John Wiley and Sons
Year: 1967
Tongue: English
Weight: 294 KB
Volume: 70
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040700213

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✦ Synopsis

The pH range for optimal growth of WI-38 in Eagle's minimum essential medium varies with the carbohydrate utilized. Growth with glucose occurred only in the pH range 6.8-7.4. Glucose-free galactose supported growth only if the pH of the medium was adjusted to 6.4-6.8. Supplementing the galactose medium with pyruvate extended the pH range for growth to 7.4, The addition of 0.01% glucose to the galactose medium increased growth at pH 7.0-7.4, reduced growth at pH 6.8, and completely inhibited growth at pH 6.6. This toxic effect of glucose under conditions impairing glycolysis was considered to be a contributing factor to the post-confluent growth rate decline associated with "contact inhibition.

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