The pH range for optimal growth of WI-38 in Eagle's minimum essential medium varies with the carbohydrate utilized. Growth with glucose occurred only in the pH range 6.8-7.4. Glucose-free galactose supported growth only if the pH of the medium was adjusted to 6.4-6.8. Supplementing the galactose med
Growth of the L-cell in galactose medium
β Scribed by Clarence L. Baugh; Joan M. Fitzgerald; Alfred A. Tytell
- Publisher
- John Wiley and Sons
- Year
- 1967
- Tongue
- English
- Weight
- 185 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
β¦ Synopsis
The failure of the Lcell to grow i n a medium containing galactose as the sole carbohydrate was found to be directly related to the initial pH of the medium. Excellent growth of L-M cultures in Eagle's minimum essential medium containing galactose instead of glucose was obtained by adjusting the medium to pH 6.2-6.8.
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