✦ LIBER ✦

Studies and observations on the growth of mammalian cells in agitated fluid medium

✍ Scribed by Cherry, William R. ;Hull, Robert N.

Publisher: Wiley (John Wiley & Sons)
Year: 1960
Weight: 959 KB
Volume: 2
Category: Article
ISSN: 0368-1467
DOI: 10.1002/jbmte.390020304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Fourteen mammalian cell strains were studied in the ‘stirrer culture’. Fair to excellent growth was obtained with twelve of these strains while two strains failed to show evidence of proliferation. Growth curves, maximal population densities, and recovery following subculture of various cell strains, cultivated in this system, were described. Factors effecting the growth of cells under these conditions were outlined. Growth of cells in methods other than the ‘stirrer culture’ were reported. The practical importance of fluid suspension culture of mammalian cells, the potentialities and the problems involved in scaling such cultures to industrial size were discussed.

📜 SIMILAR VOLUMES

Further observations on the parafollicul

Further observations on the parafollicular cells of the mammalian thyroid

✍ Nonidez, Jos� F. 📂 Article 📅 1932 🏛 John Wiley and Sons 🌐 English ⚖ 867 KB

The growth of L-cells and Vero cells on

The growth of L-cells and Vero cells on an autoclavable MEM–peptone medium

✍ Leonard Keay 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 581 KB

## Abstract The growth of L‐60TM cells (a suspension culture adapted L‐cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has been determined. It has been found that MEM autoclaved with 0.5% bactopeptone

The effects of phytohemagglutinin on the

The effects of phytohemagglutinin on the growth and histochemical properties of mammalian cells in tissue culture

✍ Caso, Louis V. 📂 Article 📅 1968 🏛 John Wiley and Sons 🌐 English ⚖ 699 KB

Effects of glutamine supply on growth an

Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture

✍ Nienke Vriezen; Bastiaan Romein; Karel Ch. A. M. Luyben; Johannes P. van Dijken 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 225 KB 👁 3 views

Glutamine is a major source of energy, carbon, and nitrogen for mammalian cells. The amount of glutamine present in commercial mammalian cell media is, however, not necessarily balanced with cell requirements. Therefore, the effects of glutamine limitation on the physiology of two mammalian cell lin

Alkaline phosphatase activity and the re

Alkaline phosphatase activity and the regulation of growth in transformed mammalian cells

✍ Ben-Ami Sela; Leo Sachs 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 615 KB

## Abstract The relationship between alkaline phosphatase activity and cell growth has been studied in hamster cells transformed by different carcinogens. About 90% of normal hamster embryo cells were constitutively positive for alkaline phosphatase activity (AP^+^). However, there were no AP^+^ ce

Studies on the penetration of mammalian

Studies on the penetration of mammalian cells by deoxyribonucleoside-5′-phosphates

✍ M. Anwar Waqar; Robert L. Taber; Joel A. Huberman 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 635 KB

## Abstract We have tested the ability of [5′‐^32^P]‐deoxyribonucleoside monophosphates (dNMPs) to penetrate living mouse fibroblast L cells and human HeLa cells. Under the conditions of our experiments, small numbers of apparently intact dNMP molecules appeared to penetrate into the interior of L