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Renaturation of DNA in the presence of ethidium bromide

✍ Scribed by James R. Huttpm; James G. Wetmur

Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
611 KB
Volume
11
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The rate of renaturation of T2 DNA has been studied as a fuction of ethidium bound per nucleotide of denatured DNA. The Binding constants and number of binding sites for ethidium have been determined by spectral titration for denatured DNA at 55, 65, and 75Β°C and for native DNA at 65Β°C in 0.4__M__ Na^+^. The rate of renaturation of T2 DNA was found to be independentof ethidium binding up to 0.03 moles per mole of nucleotide. Above 0.03 moles, the rate drops off precipitously approaching zero at 0.08 and 0.06 moles bound ethidium per nucleotide at 65Β°C respectively. A study was also made of the use of bound ethidium fluorescence as a probe for monitoring DNA renaturation reactions.

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