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Electrochemical Studies of the Intercalation of Ethidium Bromide to DNA

✍ Scribed by Tzyh-Chyang Tang; Hsuan-Jung Huang

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
247 KB
Volume
11
Category
Article
ISSN
1040-0397

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✦ Synopsis

Ethidium bromide in a concentration range of 5.7 nM to 0.7 mM was determined amperometrically with an electrochemically activated glassy carbon electrode. From the relationship derived for the intercalation of EB to DNA and the equilibrated concentration of free ethidium bromide determined, the binding constant of the EB-DNA intercalation was determined. The binding constants found agree very well with those reported in the literature. The linear relationship found for EB-DNA intercalation was developed as a new procedure for DNA analysis. With the developed method, a detection limit of 0.4 mM and a linear dynamic range of 0.4 to 107 mM for dsDNA analysis can be obtained optimistically.

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