The effect of ethidium bromide on the liquid crystalline phases of aqueous DNA
โ Scribed by Prof. Giovanni Gottarelli; Gian Piero Spada; Paolo Mariani; Monica Miranda De Morais
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 632 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0899-0042
No coin nor oath required. For personal study only.
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## Abstract The rate of renaturation of T2 DNA has been studied as a fuction of ethidium bound per nucleotide of denatured DNA. The Binding constants and number of binding sites for ethidium have been determined by spectral titration for denatured DNA at 55, 65, and 75ยฐC and for native DNA at 65ยฐC
Ethidium bromide in a concentration range of 5.7 nM to 0.7 mM was determined amperometrically with an electrochemically activated glassy carbon electrode. From the relationship derived for the intercalation of EB to DNA and the equilibrated concentration of free ethidium bromide determined, the bind
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