✦ LIBER ✦

A simplified microassay of DNA and RNA using ethidium bromide

✍ Scribed by G.J. Boer

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 436 KB
Volume: 65
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90507-2

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✦ Synopsis

The ethidium bromide method of Karsten and Wollenberger (3) has been modified allowing determination of DNA and RNA in small samples of tissue homogenates (IO-30 pg wet weight) or in small pieces of frozen-dried tissue sections (2-5 pg dry weight). The detection limit for DNA ranges between 5 and 10 ng, depending on the origin of the tissue, and for RNA is twice as high. The present micromethod offers a good fluorimetric alternative for the more laborious diaminobenzoic acid DNA assay of Kissane and Robins (4).

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