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Relationships between steroid binding and elevation of alkaline phosphatase in HeLa cells

✍ Scribed by Melnykovych, George; Bishop, Carole F.

Book ID
123382620
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
426 KB
Volume
177
Category
Article
ISSN
0304-4165

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## Abstract Parallel changes in the enzyme activities of Ca^2+^ ATPase and alkaline phosphatase were observed in HeLa cells. Both enzymes were inhibited to a similar degree by L‐phenylalanine, L‐tryptophan, and L‐leucine, while being relatively resistant to L‐homoarginine. Exposure to heat (56Β°C, 6

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Reduction in alkaline phosphatase activity was observed when HeLa S3 cells were grown in Puck's medium containing high concentrations of human serum. This effect was not seen with the enzyme of Chang liver 8A cells. The induction of increased alkaline phosphatase in HeLa S3 by prednisolone or by osm