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Similarities between alkaline phosphatase and CA2+ ATPase activities in HeLa cells

✍ Scribed by Prem Brahmacupta; George Melnykovych

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
526 KB
Volume
105
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Parallel changes in the enzyme activities of Ca^2+^ ATPase and alkaline phosphatase were observed in HeLa cells. Both enzymes were inhibited to a similar degree by L‐phenylalanine, L‐tryptophan, and L‐leucine, while being relatively resistant to L‐homoarginine. Exposure to heat (56°C, 60°C, and 65°C) resulted in a loss of both enzyme activities. Both alkaline phosphatase and Ca^2+^ ATPase, when treated with EGTA, required Ca^2+^ for the restoration of activity. Cells grown in the presence of agents that affect alkaline phosphatase (dexamethasone, butyric acid, and hyperosmolar NaCl) showed similar changes in the activities of both enzymes.

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