Most of the published work on the chemical synthesis of oligonucleotides\* has involved the us8 of intermediates with unprotected lnternucleotide phosphodiester linkages. Such intermediates can usually be isolated only on a comparatively small scale and are sometimes difficult to obtain pure. Furthe
Rapid synthesis of oligonucleotides by the phosphotriester method
โ Scribed by V.A. Efimov; O.G. Chakhmakhcheva
- Book ID
- 118222546
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 852 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0300-9084
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The present phosphotriester strategy for the (solution-phase) synthesis of the 3'-5' internucleotide bond of DNA is basically a two-step process' (Fig. ). We have modified and simplified this process by coupling together these up-to-now generally uncoupled reactions\* into one continuous and abbrevi
4,6-Dinitro-l-(mesitylene-2-sulphonyloxy)-benzotriazole [DMBT, (@)I, l-(mesitylene-2-sulphonyloxy)-4-nitro-6-trifluoromethylbenzotriazole (@) and 1-(mesitylene-2-sulphonyl)-4nitro-1,2,3-triazole [iso-MSNT, (211 are more reactive condensing agents in the phosphotriester approach to oligonucleotide sy