Perhaps the most serious problem which we have encountered in the synthesis of oligonucleotides by the phosphotriester approach with phenyl and other aryl protecting groups'. is the internucleotide cleavage which accompanies the removal of the latter protecting groups. For example, in a recent stu
Internucleotide cleavage during unblocking in oligonucleotide synthesis by the phosphotriester approach
β Scribed by J.H. van Boom; P.M.J. Burgers; P.H. van Deursen; R. Arentzen; C.B. Reese
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- French
- Weight
- 185 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Most of the published work on the chemical synthesis of oligonucleotides\* has involved the us8 of intermediates with unprotected lnternucleotide phosphodiester linkages. Such intermediates can usually be isolated only on a comparatively small scale and are sometimes difficult to obtain pure. Furthe
4,6-Dinitro-l-(mesitylene-2-sulphonyloxy)-benzotriazole [DMBT, (@)I, l-(mesitylene-2-sulphonyloxy)-4-nitro-6-trifluoromethylbenzotriazole (@) and 1-(mesitylene-2-sulphonyl)-4nitro-1,2,3-triazole [iso-MSNT, (211 are more reactive condensing agents in the phosphotriester approach to oligonucleotide sy
It has been observed that the use of U6-protected deoxyguanosine phosphoramidites leads to a significant improvement in the automated synthesis of oligonucleotides on an insoluble support. The introduction of deoxyribonucleoside-3'-phosphoKamidite derivatives by Caruthers and Beaucagel has lead to
One of the major problems,in oligonucleotide synthesis is the design of suitable protecting groups for the reactive hydroxyl and amino functions of nucleosides and nucleotides. Ideally, these protecting groups should satisfy the following conditions: 1) they should be stable under the reaction cond