Protoplasts were isolated from hypocotyls of 7-d-old seedlings of three genotypes of Brassica carinata after enzymatic digestion in cellulase R-10 (0.5%) and pectolyase Y-23 (0.025%). The protoplasts were stabilized with 0.4 M mannitol used as osmoticum, and were cultured in darkness in Kao's liquid
Rapid and high frequency shoot regeneration from hypocotyl protoplasts ofBrassica nigra
โ Scribed by S. B. Narasimhulu; P. B. Kirti; Shyam Prakash; V. L. Chopra
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 329 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0167-6857
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A simple protocol has been developed for high frequency protoplast regeneration via somatic embryogenesis in B. napus. Protoplasts isolated from hypocotyl tissue of 8-12 day old seedlings of Brassica napus ISN706 (AACC) when cultured in KM(A) medium resulted in divisions with a, frequency ranging fr
An efficient tissue culture system for high frequency of plant regeneration from hypocotyl explants of Brassica carinata was developed via manipulation of culture medium and selection of explants. Explants grown on medium containing combinations of 2mg 1 ~ BA and 0.01 mg 1 i NAA or 4mg 1 I kinetin a
Protoplasts derived from hypocotyls of seedlings grown on half-strength MS medium containing 1% sucrose were cultured at a density of 5 x 104 ml ~ in Kao's medium supplemented with 1.0 mg 1 ~ 2,4-D, 0.1 mg 1l NAA and 0.5mgl ~ zeatin riboside. After three days of culture in darkness at 25 + IยฐC, cult