High frequency of plant regeneration from hypocotyl explants ofBrassica carinataA. Br.

✍ Scribed by Mei-Zhu Yang; Shi-Rong Jia; Eng-Chong Pua

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 265 KB
• Volume: 24
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00039734

✦ Synopsis

An efficient tissue culture system for high frequency of plant regeneration from hypocotyl explants of Brassica carinata was developed via manipulation of culture medium and selection of explants. Explants grown on medium containing combinations of 2mg 1 ~ BA and 0.01 mg 1 i NAA or 4mg 1 I kinetin and 0.01 mg 1-J 2,4-D regenerated shoots at 100% frequency. High frequency shoot regeneration occurred only from explants originating from 6 to 7-day-old but not younger or older seedlings. Explants showed higher regeneration capacity at the distal end than the proximal end, and the upper segment was more regenerative than the lower segment of hypocotyl. Regenerants were rooted on half-strength growth regulator-free medium, acclimatized and developed into normal, fertile plants.