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High frequency somatic embryogenesis and plantlet regeneration from hypocotyl protoplast cultures ofBrassica napus

✍ Scribed by Dwarkesh S. Parihar; Satish C. Maheshwari; Paramjit Khurana

Publisher: Springer
Year: 1995
Tongue: English
Weight: 245 KB
Volume: 42
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00037689

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✦ Synopsis

A simple protocol has been developed for high frequency protoplast regeneration via somatic embryogenesis in B. napus. Protoplasts isolated from hypocotyl tissue of 8-12 day old seedlings of Brassica napus ISN706 (AACC) when cultured in KM(A) medium resulted in divisions with a, frequency ranging from 30-35%. Regeneration of plantlets was possible by both organogenesis and embryogenesis. Nearly 80% of the call transferred on to MS medium supplemented with 5.0 mg 1-1 2iP, 0.1 mg 1-1 NAA, 0.001 mg 1 -~ GA3, 0.5 g 1 -~ PVP and 0.5 g 1-1 MES displayed somatic embryogenesis. The somatic embryos developed into normal plantlets, and also displayed secondary, repetitive embryogenesis.

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