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Somatic embryogenesis and high frequency plantlet regeneration in callus cultures ofThevetia peruviana

✍ Scribed by Anjani Kumar

Publisher
Springer
Year
1992
Tongue
English
Weight
678 KB
Volume
31
Category
Article
ISSN
0167-6857

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✦ Synopsis

Plantlet regeneration through somatic embryogenesis has been achieved in the apocynaceous medicinal tree Thevetia peruviana L. Calluses obtained by culturing young leaf discs on MS medium containing 9 IxM 2,4-dichlorophenoxyacetic acid and 4.6 IxM kinetin, when subjected to reduced levels of the growth regulators followed by higher cytokinin treatment, produced numerous somatic embryos. Somatic embryos developed into complete plantlets on a medium devoid of growth regulators. An average of 40-50 plantlets were obtained from 50 mg of embryogenic callus. Survival of transplants was 60% under glasshouse conditions.

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