Rapid and efficient plant regeneration f
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S. B. Narasimhulu; P. B. Kirti; S. Prakash; V. L. Chopra
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Article
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1992
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Springer
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English
โ 345 KB
Protoplasts were isolated from hypocotyls of 7-d-old seedlings of three genotypes of Brassica carinata after enzymatic digestion in cellulase R-10 (0.5%) and pectolyase Y-23 (0.025%). The protoplasts were stabilized with 0.4 M mannitol used as osmoticum, and were cultured in darkness in Kao's liquid