๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Plant regeneration from hypocotyl-derived protoplasts ofBrassica juncea(L.) Czern and Coss

โœ Scribed by P. B. Kirti; V. L. Chopra

Publisher
Springer
Year
1989
Tongue
English
Weight
245 KB
Volume
7
Category
Article
ISSN
0721-7714

No coin nor oath required. For personal study only.

๐Ÿ“œ SIMILAR VOLUMES

Rapid and efficient plant regeneration f
Rapid and efficient plant regeneration from hypocotyl protoplasts ofBrassica carinata
โœ S. B. Narasimhulu; P. B. Kirti; S. Prakash; V. L. Chopra ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 345 KB

Protoplasts were isolated from hypocotyls of 7-d-old seedlings of three genotypes of Brassica carinata after enzymatic digestion in cellulase R-10 (0.5%) and pectolyase Y-23 (0.025%). The protoplasts were stabilized with 0.4 M mannitol used as osmoticum, and were cultured in darkness in Kao's liquid

Plant regeneration from stem cortex expi
Plant regeneration from stem cortex expiant and protoplast cultures ofBrassica juncea(mustard)
โœ A.-C. Bonfils; A. Sproule; J. A. Webb; W. A. Keller ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 392 KB

Plant regeneration from stem cortex explants of 13 genotypes of Brassica juncea was assessed. Regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (Blaze), whereas no shoots were obtained from less responsive lines (Zeml, Vniimk

Rapid plant regeneration through organog
Rapid plant regeneration through organogenesis and somatic embryogenesis from cultured protoplasts ofBrassica juncea
โœ P. B. Kirti; V. L. Chopra ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 211 KB

Protoplasts derived from hypocotyls of seedlings grown on half-strength MS medium containing 1% sucrose were cultured at a density of 5 x 104 ml ~ in Kao's medium supplemented with 1.0 mg 1 ~ 2,4-D, 0.1 mg 1l NAA and 0.5mgl ~ zeatin riboside. After three days of culture in darkness at 25 + IยฐC, cult