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Plant regeneration from stem cortex expiant and protoplast cultures ofBrassica juncea(mustard)

โœ Scribed by A.-C. Bonfils; A. Sproule; J. A. Webb; W. A. Keller

Publisher
Springer
Year
1992
Tongue
English
Weight
392 KB
Volume
11
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

Plant regeneration from stem cortex explants of 13 genotypes of Brassica juncea was assessed. Regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (Blaze), whereas no shoots were obtained from less responsive lines (Zeml, Vniimk351). Blaze was chosen for B. juncea stem cortex protoplast isolation. After one week of culture, 11-14% of the cells had divided, and about 0.002% produced 1-2 mm colonies within 6 weeks. Up to 7% of these colonies gave rise to shoots upon transfer to plant regeneration medium.

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